FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_input_bzip1_180_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_input_bzip1_180_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27187083
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG680080.2501482045720021RNA PCR Primer, Index 39 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC176000.062.038644
GCCGTCT174250.061.9687149
GTATGCC176250.061.67656745
TATGCCG176550.061.532846
TGCCGTC176500.061.49147448
ATGCCGT176850.061.46731647
CCGTCTT181450.059.50999550
TCTCGTA174400.059.36351841
ATCTCGT177350.058.2401340
TACATCT184200.056.0377737
TGCTTGA189000.055.90987458
GCTTGAA190000.055.9062359
ATACATC192150.054.0966136
CTATACA209600.052.45808834
CCTATAC212100.051.9860733
CTTGAAA205100.051.79036360
ACCTATA225550.049.00840432
TTCTGCT233000.045.0260555
TCGTATG252000.044.09501643
CTCGTAT247350.043.3759142