Basic Statistics
Measure | Value |
---|---|
Filename | HJJJKBGX5_n01_input_bzip1_180_pchx_r1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 27187083 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 68008 | 0.2501482045720021 | RNA PCR Primer, Index 39 (100% over 43bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 17600 | 0.0 | 62.0386 | 44 |
GCCGTCT | 17425 | 0.0 | 61.96871 | 49 |
GTATGCC | 17625 | 0.0 | 61.676567 | 45 |
TATGCCG | 17655 | 0.0 | 61.5328 | 46 |
TGCCGTC | 17650 | 0.0 | 61.491474 | 48 |
ATGCCGT | 17685 | 0.0 | 61.467316 | 47 |
CCGTCTT | 18145 | 0.0 | 59.509995 | 50 |
TCTCGTA | 17440 | 0.0 | 59.363518 | 41 |
ATCTCGT | 17735 | 0.0 | 58.24013 | 40 |
TACATCT | 18420 | 0.0 | 56.03777 | 37 |
TGCTTGA | 18900 | 0.0 | 55.909874 | 58 |
GCTTGAA | 19000 | 0.0 | 55.90623 | 59 |
ATACATC | 19215 | 0.0 | 54.09661 | 36 |
CTATACA | 20960 | 0.0 | 52.458088 | 34 |
CCTATAC | 21210 | 0.0 | 51.98607 | 33 |
CTTGAAA | 20510 | 0.0 | 51.790363 | 60 |
ACCTATA | 22555 | 0.0 | 49.008404 | 32 |
TTCTGCT | 23300 | 0.0 | 45.02605 | 55 |
TCGTATG | 25200 | 0.0 | 44.095016 | 43 |
CTCGTAT | 24735 | 0.0 | 43.37591 | 42 |