Basic Statistics
Measure | Value |
---|---|
Filename | HJJJKBGX5_n01_ev_5_pchx_r2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13717937 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 117550 | 0.8569072740310734 | RNA PCR Primer, Index 24 (100% over 43bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG | 15841 | 0.11547654723884503 | RNA PCR Primer, Index 24 (100% over 43bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 37080 | 0.0 | 62.000465 | 45 |
TCTCGTA | 34215 | 0.0 | 61.929066 | 41 |
CGTATGC | 37135 | 0.0 | 61.750935 | 44 |
GTAGCAT | 42400 | 0.0 | 61.423466 | 35 |
TAGCATC | 39260 | 0.0 | 61.239403 | 36 |
GGTAGCA | 44390 | 0.0 | 60.505123 | 34 |
TCGTATG | 37905 | 0.0 | 59.75932 | 43 |
CTCGTAT | 36125 | 0.0 | 59.571537 | 42 |
CGGTAGC | 45525 | 0.0 | 59.330524 | 33 |
CTTGAAA | 33055 | 0.0 | 59.1973 | 60 |
ACGGTAG | 45735 | 0.0 | 59.179012 | 32 |
CACGGTA | 46095 | 0.0 | 58.709557 | 31 |
TGCTTGA | 31600 | 0.0 | 58.66943 | 58 |
TATGCCG | 38775 | 0.0 | 58.489452 | 46 |
GCTTGAA | 33125 | 0.0 | 58.46794 | 59 |
ATCTCGT | 37685 | 0.0 | 58.23152 | 40 |
TGAAAAA | 41445 | 0.0 | 57.7767 | 62 |
ATGCCGT | 38420 | 0.0 | 57.045475 | 47 |
CCGTCTT | 35475 | 0.0 | 56.60762 | 50 |
GCATCTC | 39800 | 0.0 | 56.316315 | 38 |