FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_ev_5_pchx_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_ev_5_pchx_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13717937
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1175500.8569072740310734RNA PCR Primer, Index 24 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG158410.11547654723884503RNA PCR Primer, Index 24 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC370800.062.00046545
TCTCGTA342150.061.92906641
CGTATGC371350.061.75093544
GTAGCAT424000.061.42346635
TAGCATC392600.061.23940336
GGTAGCA443900.060.50512334
TCGTATG379050.059.7593243
CTCGTAT361250.059.57153742
CGGTAGC455250.059.33052433
CTTGAAA330550.059.197360
ACGGTAG457350.059.17901232
CACGGTA460950.058.70955731
TGCTTGA316000.058.6694358
TATGCCG387750.058.48945246
GCTTGAA331250.058.4679459
ATCTCGT376850.058.2315240
TGAAAAA414450.057.776762
ATGCCGT384200.057.04547547
CCGTCTT354750.056.6076250
GCATCTC398000.056.31631538