FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_ev_5_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_ev_5_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22608869
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC46000.025.1240545
TCTCGTA42100.024.41955841
CGTATGC48650.023.75557744
AGATCTC54300.019.12379838
CTTGAAA61350.018.38813860
TCGTATG70700.017.61535543
CTCGTAT68250.016.88291542
TGCTTGA67150.016.69709858
TGAAAAA75200.015.001492562
GCTTGAA78100.014.66532259
TATGCCG91300.013.26291646
ATCTCGT80750.013.20138740
TTCTGCT105550.011.1781955
CCGTCTT112850.010.85251550
ATGCCGT111950.010.66239247
GAAAAAA115500.010.51393563
CAGATCT105050.010.31197937
AGTCACG111850.010.271296528
TCTGCTT128400.09.18892656
GTCACGA139200.08.50103829
Produced by FastQC (version 0.11.2)