FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_ev_180_pchx_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_ev_180_pchx_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36018134
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1532230.425405158412704TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC302400.062.4717846
GTATGCC301200.062.4114247
GCCGTCT298450.062.17769251
ATGCCGT302000.062.12051849
TATGCCG302600.061.96314248
TGCCGTC303250.061.58030350
TCTCGTA302750.061.1118343
ATCTCGT304350.060.77921742
CCGTCTT305750.060.72752
GCTTGAA312100.058.83927561
TGCTTGA311200.058.73221660
ACTGATA331800.057.85376434
CTTGAAA325500.056.60771662
CACACTG341950.056.066231
ACACTGA342500.056.026332
GTCACAC358850.053.42591529
TATCTCG346350.053.41881641
TCACACT359100.053.4175430
TTCTGCT373000.049.2233657
TATATCT386600.048.0449639
Produced by FastQC (version 0.11.2)