FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_ev_180_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_ev_180_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23506930
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC51100.030.98809447
TCTCGTA50550.030.02856643
CGTATGC53250.029.86650346
AGTCAAC56750.028.39028734
AACAATC66850.022.75856638
TCACAGT71350.022.67801330
TCGTATG70600.022.52678945
CTCGTAT67950.022.44072244
ACAATCT71850.021.0787839
CACAGTC79100.020.71765131
CAGTCAA82550.020.26956233
CTTGAAA77700.019.89132962
TGCTTGA81150.018.79067460
AGTCACA95150.017.3681628
GCTTGAA92700.016.78438461
TGAAAAA97500.016.59491364
ATCTCGT92400.016.5773142
TATGCCG102150.016.24462748
GTCACAG112600.014.67652729
ATGCCGT119350.014.16370149
Produced by FastQC (version 0.11.2)