FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_nlp7_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_nlp7_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26947381
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2407010.8932259502324178Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA1733740.6433797777973302No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1677120.622368459480348Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC1635490.6069198338792181No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG1453180.5392657638974266No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC1249160.46355525236385675No Hit
ACCCTTATTCCACTGCACCGGCGCGGAACTGGATGAGTTCGGTCGCCGCCACCGGCATGACGCCGAGTGCCACTG974370.36158244840194304No Hit
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCTTCTG898360.33337562563129974RNA PCR Primer, Index 44 (100% over 39bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCTTCTG833880.3094475118008685RNA PCR Primer, Index 44 (100% over 39bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA661900.24562683846715938No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA440250.16337394717505202No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATACGACTCACTATAGGGCAGCGTGGTCG374010.13879270864949733No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTCG337350.12518841812493764No Hit
GGTCGCGGCCGAGGATCGATGGATGTCCCCCCTCTGGGGGCGGCGGGAGTTTTTGGGCATGCTTGCTGCTTCGGC316140.1173175233615467No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCCCGCTGCCCTATAGTGA283670.10526811492367291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTTA329500.066.705061
CCCTTAT336750.065.381782
CCTTATT338900.064.921273
TCCACTG343300.063.9599089
TATTCCA343600.063.894656
TTATTCC343750.063.8760955
CTTATTC346000.063.4804274
CACTGCA347050.063.3916811
ATTCCAC351900.062.448617
ACTGCAC364950.060.34829712
TGCTGTA67300.059.9239113
TGCCACT134350.059.4961768
TTCCACT374050.058.6847348
TGCACCG374700.058.6393314
CTGCACC380300.057.89367713
GATGAGT141750.057.70689432
GTCACAA122150.057.6742921
TGAGTTC141900.057.5971634
GCCACTG141000.056.8613169
GGTCACA125150.056.51230220
Produced by FastQC (version 0.11.2)