FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_nlp7_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_nlp7_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20702986
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3517311.698938500948607Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2452981.1848435776365787Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA860050.41542316649395405No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG759570.3668891047890387No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC338700.16359958896750448No Hit
AAGATGGTTGGCATCGAGGGCGGCGAGAATGCGCTGGCGCCTGACGTCGCCGTCGTCATTGCGCGCTGCCGCCAT224550.10846261500635705No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC219290.10592191870293494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGTA207750.063.73295213
ACCCTTA114800.063.627381
GAATCCA216750.060.7773741
CCCTTAT122500.059.9379232
GCTGTAG221400.059.7568714
AGATGGT267200.059.4905362
CTGTAGT163350.059.3454315
ATGGTTG271600.058.239934
CACTGCA126250.058.20451411
TTATTCC126150.058.1275375
AATCCAC227800.057.950532
TTCCACT126950.057.809458
GTTGGCA276250.056.94327
CTAATAC8757850.056.9379161
CTTATTC129250.056.7595184
GATGGTT280950.056.3498883
TGTAGTC125800.056.2725816
ACTGCAC130550.056.20757712
AAGATGG286200.055.6736151
TAATACG8961700.055.5655062