Basic Statistics
Measure | Value |
---|---|
Filename | HJJJKBGX5_n01_damid_nlp7_r1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20702986 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 60 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA | 351731 | 1.698938500948607 | Illumina Multiplexing PCR Primer 2.01 (100% over 33bp) |
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA | 245298 | 1.1848435776365787 | Illumina Multiplexing PCR Primer 2.01 (100% over 33bp) |
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA | 86005 | 0.41542316649395405 | No Hit |
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG | 75957 | 0.3668891047890387 | No Hit |
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC | 33870 | 0.16359958896750448 | No Hit |
AAGATGGTTGGCATCGAGGGCGGCGAGAATGCGCTGGCGCCTGACGTCGCCGTCGTCATTGCGCGCTGCCGCCAT | 22455 | 0.10846261500635705 | No Hit |
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC | 21929 | 0.10592191870293494 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCTGTA | 20775 | 0.0 | 63.732952 | 13 |
ACCCTTA | 11480 | 0.0 | 63.62738 | 1 |
GAATCCA | 21675 | 0.0 | 60.777374 | 1 |
CCCTTAT | 12250 | 0.0 | 59.937923 | 2 |
GCTGTAG | 22140 | 0.0 | 59.75687 | 14 |
AGATGGT | 26720 | 0.0 | 59.490536 | 2 |
CTGTAGT | 16335 | 0.0 | 59.34543 | 15 |
ATGGTTG | 27160 | 0.0 | 58.23993 | 4 |
CACTGCA | 12625 | 0.0 | 58.204514 | 11 |
TTATTCC | 12615 | 0.0 | 58.127537 | 5 |
AATCCAC | 22780 | 0.0 | 57.95053 | 2 |
TTCCACT | 12695 | 0.0 | 57.80945 | 8 |
GTTGGCA | 27625 | 0.0 | 56.9432 | 7 |
CTAATAC | 875785 | 0.0 | 56.937916 | 1 |
CTTATTC | 12925 | 0.0 | 56.759518 | 4 |
GATGGTT | 28095 | 0.0 | 56.349888 | 3 |
TGTAGTC | 12580 | 0.0 | 56.27258 | 16 |
ACTGCAC | 13055 | 0.0 | 56.207577 | 12 |
AAGATGG | 28620 | 0.0 | 55.673615 | 1 |
TAATACG | 896170 | 0.0 | 55.565506 | 2 |