FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_gr_pdex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_gr_pdex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723215
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG50560.6991005440982281RNA PCR Primer, Index 45 (100% over 43bp)
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT16750.2316047095262128No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG13180.18224179531674536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG11750.16246897533928362RNA PCR Primer, Index 45 (100% over 43bp)
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG11450.15832083128806787No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG11430.15804428835132014No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT11020.15237515814799196No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG10580.1462912135395422RNA PCR Primer, Index 45 (100% over 43bp)
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG10510.14532331326092518No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC9210.1273480223723236No Hit
GGTCGCGGCCGAGGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTT9200.12720975090394973No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC8770.12126407776387382No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGA8590.11877519133314436No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT7530.10411841568551537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG252750.068.179171
GTCGCGG257400.066.934082
TCGCGGC272650.062.9712453
GATCATA5900.061.98137314
CGCGGCC283650.060.577864
CGGCCGA298850.057.519876
GCGGCCG300300.057.311075
GGATCAT30250.057.13729513
GGCCGAG301850.056.9556857
GATCCAT14350.056.49672714
GCCGAGG305600.056.3245248
GATCTAA6200.056.20009214
GGATCTA17750.056.1703113
GATCTTT9200.055.8734914
AGGATCT88450.055.69775812
AGGATCA81250.055.6231112
GGATCGT10000.055.54349513
GATCGTT3750.055.19850514
GGATCTT36900.055.16111413
GGATCCA38650.054.98428313
Produced by FastQC (version 0.11.2)