FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_gr_mdex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_gr_mdex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910261
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG146150.7650786986699724RNA PCR Primer, Index 47 (100% over 43bp)
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGA127380.6668198743522482No Hit
GGTCGCGGCCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGA108390.567409374949287No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG76150.3986366260945494No Hit
CCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAG66540.34832936441669493No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG64760.339011265999777No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG64600.33817368411960463No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC53230.27865302175985374No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC43710.22881689988959622No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT35660.1866760615434226No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGGCACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT33240.17400763560581511No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGACACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT30840.1614439074032292No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT27750.1452681073423998No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC24690.12924935388410275No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG23970.12548023542332695RNA PCR Primer, Index 47 (100% over 43bp)
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG23460.12281044318027745No Hit
CGGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCC22710.11888427811696936No Hit
GGTCGCGGCCGAGGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTAT21910.11469636871610739No Hit
GGTCGCGGCCGAGGATCTTTGGTTCGCGGGTCGTTCTGTTTTGGACAGGTATCGACAATGATCCTTCCGCAGGTT20970.10977557517009456No Hit
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCCTCGG19960.10448833955150631No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG19900.10417424634644167RNA PCR Primer, Index 47 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG634200.067.180931
GTCGCGG646550.065.6041952
TCGCGGC669750.063.0378463
CGCGGCC681550.061.40984
TAATACG18300.059.576712
CTAATAC18600.058.6157881
GCGGCCG733950.057.1900185
CGGCCGA754650.055.572466
ACTATAG19350.055.4479913
GATCCAT57500.055.0183914
GGATCTA39700.054.9203113
ATACGAC20000.054.689494
GATCGGT16100.054.64126214
CACTATA19650.054.6028912
GGATCTT93450.054.3419113
GGCCGAG775500.054.177857
AGGATCT205700.053.97212612
ACCTAGG33500.053.6536647
GGATCGG30400.053.22377413
CTATAGG20000.053.1284514
Produced by FastQC (version 0.11.2)