FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_gr_bzip1_pdex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_gr_bzip1_pdex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322063
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT25960.1963597801315066No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG24660.1865266632528102No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG23620.17866016974985308No Hit
GGTCGCGGCCGAGGAACTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA23220.1756345953256388No Hit
GGTCGCGGCCGAGGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTT23200.1754833166044281No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT21590.1633053795469656No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC20480.154909410519771No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG19100.14447117875623175No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGA18910.14303403090472994No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG16270.12306523970491573No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC15390.1164089759716443No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT14240.10771044950202828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG446200.067.592441
GTCGCGG451000.066.860342
TCGCGGC464200.064.78073
CGCGGCC477150.063.080384
CGGCCGA490050.061.483246
GCGGCCG495500.060.834835
GGCCGAG496000.060.755067
AGGATCT143100.059.23341412
GCCGAGG508900.059.228558
GATCATA10800.058.7753614
GGATCTT59400.058.65920313
GGATCCA60200.058.45274413
GGATCTA31100.058.3480813
GATCCAA19650.058.1121114
GATCTTA10950.057.97022214
GGATCAT52700.057.67220313
GGATCCG26750.057.64809413
CCGAGGA515400.057.6448949
AGGATCA128500.057.6408212
GGATCTG22650.057.4214813
Produced by FastQC (version 0.11.2)