FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_gr_bzip1_mdex.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_gr_bzip1_mdex.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830309
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGA172290.9413164662360289No Hit
GGTCGCGGCCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGA153250.8372903154603949No Hit
CCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAG94420.5158691783737063No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG69250.37835141497965646No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG63450.3466627766131292RNA PCR Primer, Index 48 (100% over 43bp)
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG62730.3427290146090087No Hit
GGTCGCGGCCGAGGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCT48140.2630156984421756No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC46920.2563501572685268No Hit
GGTCGCGGCCGAGGATCATTTCATTTGGAGAGGACGTCGACCCTGCAGGCGGCCGCACTAGTATGAAGAAGAACA43210.23608035583062753No Hit
GGTCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCG40900.2234595360674072No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGGCACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT35160.1920987112012234No Hit
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCCTCGG34080.18619806819504248No Hit
CGGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCC33590.18352092460890485No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG32740.17887690002070689No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGACACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT30160.16478091950594134No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGA26620.14543992298568167No Hit
GGTCGCGGCCGAGGATCAAATACAAAAAGAGGACAGCACGCCTAAAAGGGTCTTGAGACTTGTTAAATTCCTCAC25860.1412876186479988No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCGACGTCCTCTCCAAATGAAATGAACT25610.1399217290632347No Hit
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT25150.13740849222726872No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC24290.1327098320556802No Hit
GAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGA23060.12598965529864084No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT20690.11304102203507714No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT20560.11233075945099981No Hit
CCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGC19730.10779600602958297No Hit
GGTCGCGGCCGAGGATCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAG19320.10555594711056984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG594450.067.545121
GTCGCGG603850.066.262992
TCGCGGC621150.064.211613
CGCGGCC634100.062.285224
CTAATAC11700.057.2165221
TAATACG11700.056.9247062
GGATCTT81550.056.68649713
GCGGCCG698750.056.665685
CGGCCGA698750.056.6558046
GATCCAT46050.055.2873214
GGATCTA33350.055.03191413
GGCCGAG723150.054.8846057
AGGATCT176500.054.845612
CTATAGG11900.054.50165614
GGATCAT68800.054.30487413
GCCCATC29800.054.29444538
GATCATA11450.054.23327314
GATCGGT14250.054.22904614
ACCTAGG29850.053.9723547
AGGATCA169850.053.88532612
Produced by FastQC (version 0.11.2)