FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_bzip1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_bzip1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17166119
Sequences flagged as poor quality0
Sequence length75
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3560152.07393995113281Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2796961.6293490683595984Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA588290.34270413714363746No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG508050.2959608983253582No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC204770.119287300757964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC6918850.061.92231
TAATACG7106550.060.2385942
TGCTGTA77750.060.1233213
AATACGA7191900.059.390353
ATACGAC7316050.058.2151224
TACGACT7355250.057.7441145
ACGACTC7346500.057.5492446
CGACTCA7412650.056.8266227
GACTCAC7447400.056.603568
ACTCACT7525500.055.851749
CTGTAGT64800.055.74112315
CTCACTA7612450.055.0690510
TCACTAT7578950.054.93842311
CACTATA7576050.054.65205812
ACTATAG7655250.054.03269213
CTATAGG7736850.053.00933514
GAATCCA88450.052.899391
TATAGGG7649750.052.87869615
CTAATAG44550.052.8232961
ATAGGGC7569550.052.81274416
Produced by FastQC (version 0.11.2)