FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_damid_bzip1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_damid_bzip1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25919977
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3832151.4784542439987505Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3077891.1874586154146665Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC1742150.6721263680133667No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA1670220.6443755717838793No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC1563070.603036800534198No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTATGCCGTCTTC1265000.4880405565174691TruSeq Adapter, Index 19 (97% over 37bp)
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTATGCCGTCTTC1240670.47865397411425176TruSeq Adapter, Index 19 (97% over 37bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG1215430.46891631115259086No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA519790.20053644337724527No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA444200.17137360885775477No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATACGACTCACTATAGGGCAGCGTGGTCG359700.13877327128801079No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTATGCCGTCTTG310750.11988822366624785TruSeq Adapter, Index 19 (97% over 37bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTCG297700.11485349697648264No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCCCGCTGCCCTATAGTGA270830.10448697543211553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTTA318050.067.8687441
CCTTATT320400.067.612833
TATTCCA321750.067.180816
CCCTTAT324950.066.746422
CTTATTC324700.066.686584
TTATTCC324150.066.6510855
TCCACTG327450.065.94949
TGCTGTA236600.065.1478213
GAATCCA237300.064.965671
ATTCCAC334050.064.731777
TTCCACT336250.064.234938
CTGCACC338350.063.73599213
TGCACCG341150.063.1724314
CTGCTGT243900.063.12756312
AATCCAC245400.062.877812
CACTGCA346500.062.37721311
AAGATGG234300.062.336791
TCCACCA245350.062.333384
AGATGGT234100.062.198682
GATGGTT234400.061.9172973
Produced by FastQC (version 0.11.2)