FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_bzip1_5_pchx_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_bzip1_5_pchx_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22132615
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1459340.6593617609125718TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT275740.12458536869683044TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG245440.1108951653476103TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT340150.058.59160644
GTATGCC357300.058.3572747
TACGTAA382550.058.31146236
CGTATGC357200.058.28668246
TCGTATG353400.058.2593745
GTACGTA385550.058.0548535
TCTCGTA344300.057.745143
CGTACGT390950.057.13928634
ACGTAAT387350.056.9832437
ACCGTAC406200.055.08802832
TAATCTC368500.055.08567440
GTAATCT372100.054.9887339
CCGTACG415800.053.79931633
CGTAATC399400.052.67260738
CACCGTA431700.051.8023131
AATCTCG403700.050.1372941
TATGCCG414750.049.91610748
ATCTCGT403000.049.9076442
TGCTTGA361450.049.8513960
CTTGAAA375500.049.8326862
Produced by FastQC (version 0.11.2)