FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_bzip1_5_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_bzip1_5_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23344781
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC36500.020.88803945
CGTATGC36200.020.87054444
TCTCGTA32650.020.6039141
TCGTATG47450.016.28583543
CTCGTAT47400.016.15745242
TGAAAAA81200.013.38300762
AGTCACC109900.013.34194828
CTTGAAA53500.012.70318260
CAGTCAC118000.012.27978127
ACTCCAG132450.011.27870623
GAAAAAA111550.010.45312963
CTCCAGT147150.010.19887624
AACTCCA147750.010.1808122
ATCATCT99200.09.87677537
ATCTCGT83150.09.70849340
TGCTTGA64000.09.70272258
CCAGTCA168150.08.94572926
GCTTGAA79950.08.88893659
AGATCAT174750.08.7260635
TATGCCG94100.08.68874446
Produced by FastQC (version 0.11.2)