FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_bzip1_180_pchx_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_bzip1_180_pchx_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17825382
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1933701.0848014365133942TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT406030.22778193477144TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG378610.21239937522797547TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA396650.060.12577443
CGTATGC406800.060.0842346
GTATGCC405500.060.00460447
CTCGTAT407550.058.59404444
TCGTATG415850.058.57753445
TATCTCG409150.058.20454441
CTTGAAA414050.056.8831462
TGGATAT437300.056.28093337
ACGAGTG440850.056.15046732
TGCTTGA414900.055.9101660
TCACGAG446700.055.3536430
ATCTCGT432750.055.1818342
GCTTGAA425800.055.07848461
GTGGATA450250.054.84608536
TATGCCG444300.054.76450748
CACGAGT452700.054.60460331
CGAGTGG454650.054.4080433
ATATCTC441900.053.94555340
GATATCT443700.053.84348339
GGATATC449850.053.50631338
Produced by FastQC (version 0.11.2)