FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_bzip1_180_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_bzip1_180_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20116276
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTT62250.035.4696831
TCACACT71000.031.09849730
ACACTTG81250.027.55738432
GTATGCC34250.027.4981645
TCTCGTA31650.027.14121841
CTTGAAT80650.027.07742535
GTCACAC81500.027.04960829
AGTCACA86600.025.41684328
CGTATGC35600.025.19573644
TTGAATC91650.021.38074536
CACTTGA109550.021.1313333
CTCGTAT44700.020.14358142
CAGTCAC112300.019.72295227
TCGTATG45800.019.20783643
ACTCCAG122900.018.30267323
TGAAAAA86900.017.15022762
CTCCAGT136850.016.58826624
ACTTGAA140800.016.44119334
AACTCCA138750.016.31134222
TGAATCT87150.015.4383937
Produced by FastQC (version 0.11.2)