FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7911131
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT99240.12544350485411No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC79950.10106013918869503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG17600.047.6313115
CACGTCT19950.042.19379414
CAGTCAC19050.041.47182527
ACACGTC20800.040.46953213
AGTCACC22700.034.80410828
GTCTGAA25900.033.03321517
CGGGACT17800.030.2370361
GAGCACA32200.027.4281649
GTCGTAT3750.026.68112242
AGCACAC32200.026.14197310
TCGTATG6600.026.13746343
ACATGCG18000.025.4930614
CGCTATG35350.024.20408229
GTCGCTA35650.024.19318827
TATGCCG7850.024.17283646
CTCCAGT34900.024.11952824
ATGCGTG19600.024.1161166
CATGCGT19900.023.579185
TCTGAAC37200.023.4626218
GAACTCC37550.023.06032621
Produced by FastQC (version 0.11.2)