FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5585843
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT57970.10378021723847232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG8750.034.6949715
CACGTCT9900.031.3619514
CGGGACT12000.027.8899961
CTCGTAT5450.027.85445842
ACACGTC11250.027.29186613
TCGTATG5250.026.9438243
CAGTCAC10600.025.38679327
CGCTATG19700.022.94326829
GTCTGAA13500.022.7430217
TATGCCG7250.022.36651846
GTCGCTA20500.022.21463427
GTCCCTA11700.021.23028
ACTCAAT12200.021.21110335
CTTAAAC14200.020.6532571
GCGTTAC2850.020.57784367
CTACCCG16500.020.4932926
CTATAAG17900.020.43108446
CCTATTA12550.020.06665211
GGTAACT23050.020.05514169
CGTATGC8200.019.77492144
Produced by FastQC (version 0.11.2)