FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10328532
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG16200.033.85936715
TCGTATG12550.031.88896643
CTCGTAT12600.030.66775942
GTCACGT16050.028.80588229
AGTCACG16000.028.463128
TATGCCG14650.026.61122946
CGGGACT24150.025.7160721
CACGTCT21600.025.5546114
ACACGTC22900.023.95291313
CGTATGC17000.023.7441644
CAGTCAC20050.023.57417927
ACGTAGA20700.023.16816532
CACGTAG20550.022.49802431
ATGCGTG25100.022.4065916
CGTAGAG20850.022.17377333
ACATGCG24750.021.88694
CATGCGT25250.021.3169545
TTACGGG10400.020.898578
CTACCCG29250.020.2891796
CGCTATG39550.020.15194129
Produced by FastQC (version 0.11.2)