FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8519452
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13250.034.36830515
CACGTCT17650.025.99602914
ACACGTC20300.023.4523113
CGGGACT14550.021.34141
CAGTCAC18400.021.00006527
CGCTATG25750.020.76827429
GTCGCTA27600.019.37506127
GTCTGAA25950.018.21296917
GGTAACT31000.017.02687569
GCTATGA31700.016.97896830
ACCTAGG20750.016.6262657
GTGGTAA32450.016.37235667
CGGAATA16650.016.36884368
AGTCACC25350.016.33160428
CACACGT28400.016.27774212
CGCGTAT7550.015.9942711
CTACCCG18750.015.6413356
CTTAAAC23200.015.6150971
ATCGAAG41750.015.5366556
GTGGATA22050.015.3341911
Produced by FastQC (version 0.11.2)