FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11521623
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT119460.10368330920044859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14000.032.7752845
ACGTCTG17450.031.43416415
CTCGTAT15050.030.94670744
TATGCCG15850.029.60226448
CACGTCT20450.027.16018914
CGTATGC17550.027.12820446
CGGGACT24100.026.0559751
TATCTCG18200.025.21328741
GTCACAC19200.024.97786129
ACATGCG29200.024.9322814
ACACGTC23450.023.68574313
ATGCGTG31400.023.4054056
CAGTCAC21000.023.32846827
CATGCGT31200.022.8917065
AGTCACA21300.022.67615328
CGCTATG43050.021.87918729
GTCGCTA44100.021.27881827
TGCGAGT34700.020.48073411
TGCGTGC35550.020.3795687
GTATGCC24250.020.34415247
Produced by FastQC (version 0.11.2)