FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098090
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG17550.032.04097715
AGTCACG15300.032.02066828
CACGTCT19200.029.82698414
ACACGTC22850.025.51557413
CTCGTAT7450.025.47064242
CAGTCAC20800.024.21701627
TAGCATC21450.022.51847636
GGTAGCA22850.022.19548434
CGGGACT20000.022.0817031
ACATGCG21500.021.9854154
TCGTATG7900.021.83592843
ATGCGTG22750.021.2325236
CGCTATG33000.021.11946329
CATGCGT23500.020.7015955
GTCGCTA34750.020.15451427
GTCTGAA28700.020.07381217
GTCACGG27350.019.17483729
GTCCCTA19250.018.817778
TGCGAGT25050.018.73002811
GCTATGA38400.018.23928630
Produced by FastQC (version 0.11.2)