FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14306999
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT188690.1318864983495141No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC155060.10838052061092615No Hit
GTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCA147800.10330608117048165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG19850.036.6709815
CTCGTAT15550.036.1643442
TCGTATG16300.034.49997343
CGGGACT36900.033.5671271
CACGTCT22150.033.48615314
TATGCCG18150.031.1736446
CACTATA20950.030.13780831
TAATATC22350.027.47773736
ATGCGTG36800.027.3777566
ACACGTC27100.027.24228513
CATGCGT37450.027.0866475
CGTATGC20650.026.8981944
ACATGCG37850.026.6179874
AGTCACT24450.025.82251728
CAGTCAC24900.025.35566927
CGCTATG63300.025.18157229
GTCGCTA64150.025.115627
TGCGTGC42650.023.8625687
TATCTCG25350.023.27347439
GCTATGA70650.022.75709230
Produced by FastQC (version 0.11.2)