FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bw_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bw_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14062294
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT172970.1230026907416386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG19300.032.53204315
CGGGACT31050.030.4461611
CACGTCT23000.028.04898814
CGCTATG57950.026.256129
GTCGCTA59000.025.72943927
GCTATGA66300.022.89729930
GGTAACT65700.022.84188569
GTGGTAA68450.022.02492767
ATCGAAG72850.021.7392836
ACACGTC30050.021.69797513
CTTAAAC39300.021.6844271
CTACCCG43200.021.3250276
ATGCGTG32250.021.0765046
CTATAAG42300.021.04299246
CCCTGTG71400.020.872963
AGTCACT24800.020.58935728
ACATGCG32450.020.2021644
ACCTATA42700.019.958656
AGCAACG77500.019.89749521
TATCCCT79650.019.79387360
Produced by FastQC (version 0.11.2)