FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_4bs_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_4bs_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5777123
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT62140.1075621896919972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG8100.038.7595728
CTCGTAT8950.033.92323342
ACGTCTG11100.032.63342315
TCGTATG9750.032.20109643
GTCACGA10500.030.22933629
CGTATGC10400.028.86155744
CGGGACT12950.027.440611
CACGTCT13750.027.34836214
TATGCCG11750.026.13257846
ACACGTC14200.025.26715313
CAGTCAC14200.021.86637327
CGCTATG23900.018.9105229
CTACCCG17700.018.9077516
CACGATC17300.018.54671331
GTCGCTA25100.018.14357227
AGCACAC21700.018.12441310
GTATGCC17200.018.05356445
GTCTGAA21100.017.98483717
ATGCCGT17950.017.49069447
CGCGAAT13250.017.4455761
Produced by FastQC (version 0.11.2)