FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6416405
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG11300.036.02423515
CACGTCT13150.030.69432314
CGCTATG14500.028.55345229
GTCGCTA15000.027.60059427
ACACGTC16050.026.00848413
CGGAATA11750.023.49023234
ATATCGG6900.023.00049438
TCGGAAG17900.022.9366053
ATCGGAA18000.022.809182
GGTAACT18050.022.74439869
ACGGGTA10300.019.7628692
CACACGT22650.018.58216712
TGGTAAC23550.017.72552968
GCTATGA24350.017.71154230
CTTAAAC15850.017.6315671
CCCTGTG23450.017.50674663
GTGGTAA24500.017.17902267
GTCTGAA25400.016.7058817
ATCGAAG28850.016.2642886
AGCAACG26550.015.98264721
Produced by FastQC (version 0.11.2)