FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8917252
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG15550.036.16283415
CACGTCT18100.031.06859414
CTCGTAT11850.025.91187542
ACACGTC22050.025.50273313
TCGTATG13000.020.70038643
CGGAATA18500.020.5128668
CGCTATG26200.020.27968629
GTTAGTT25350.020.1430641
GTCGCTA26350.019.77065527
CGGGACT12000.019.5510221
CAGTCAC20750.019.28699927
GTCTGAA30050.018.94282317
TATGCCG13600.018.51862346
CGTATGC13650.018.45089344
ATCGGAA31500.017.8532142
GTCCCTA20100.017.1641128
GGTAACT30200.017.13521669
CACACGT34150.016.76969112
TCGGAAG33900.016.691233
ACTCACG22250.016.59029863
Produced by FastQC (version 0.11.2)