FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15186947
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT26650.034.30755229
TCGTATG27750.033.32071345
ACGTCTG30750.033.09596615
AGTCACG28200.032.42131828
CTCGTAT27600.031.87587744
CGTATGC32250.029.6334746
CACGTCT38150.027.39972714
TATGCCG33650.027.27248648
CACGTGA34500.026.40142331
TCACGTG36700.025.38275530
TGAAACG37100.025.20158435
ACACGTC43500.024.26771513
GAAACGA40650.023.17030136
CAGTCAC43350.021.40875227
CACACGT51450.020.04866212
ATGCCGT48600.019.45102549
GTATGCC50050.019.43916147
CGGGACT25100.018.9687751
CGCTATG43950.018.91904429
AAACGAT50150.018.50605437
Produced by FastQC (version 0.11.2)