FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18188240
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT200860.11043399471306733No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC195540.10750902781137701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT29150.037.9926329
ACGTCCG29800.037.16423832
AGTCACG30100.036.9071328
CTCGTAT25550.036.72864544
TCACGTC30800.036.1816430
TCGTATG24950.035.26222245
ACGTCTG35050.034.64598515
CGCACAT33950.032.21492437
CCGCACA35450.032.01979436
CGTATGC30100.030.60379246
TATGCCG29600.029.95493148
ACACGTC41550.029.80756213
CACGTCT42150.029.2194814
CGGGACT48600.029.1775741
CAGTCAC40550.028.16164827
ACATGCG46250.024.3942814
CATGCGT46950.023.9572185
ATGCGTG48100.023.4561676
GTCTGAA53450.022.84835817
ATGCCGT38750.022.34762249
Produced by FastQC (version 0.11.2)