FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20253186
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37050.033.98666415
CACGTCT47500.027.09076514
ACACGTC48000.026.66469213
CTCGTAT26100.026.43715944
TCGTATG24800.026.15437545
CGTATGC30350.023.07628446
GTCACAT50200.023.02380629
CGGGACT33150.022.584981
CAGTCAC52250.022.38372227
TATGCCG30400.022.356948
TATCTCG37000.022.19336141
AGTCACA52150.022.02971328
ACATTCC56750.020.24499332
GTCTGAA66550.019.23218517
CGCTATG60800.018.66931529
GTCGCTA60900.018.58127227
CACACGT71250.018.15725912
ACATGCG39800.017.8581434
CACATTC71050.016.36458831
CGCGAAT47400.016.1591381
Produced by FastQC (version 0.11.2)