FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16314446
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG27900.032.5196315
CGGGACT34850.028.9085351
ACACGTC36100.026.08868613
CACGTCT35050.025.88605114
GTCGCTA45800.021.84532727
CTACCCG44450.021.5793826
CGCTATG47950.021.0826429
CAGTCAC38600.020.64670427
CTTAAAC38750.019.8554231
AGTCACA39350.019.81486328
GTCACAT40600.019.54554729
GGTAACT53900.018.68926469
GTCTGAA49500.018.6777717
TACCCGC55550.018.56967
GGACTAA38850.018.20375362
CGCGATA51200.017.92356525
CTATAAG41300.017.87674546
GTCCCTA36600.017.6270078
ACCTATA41200.017.586856
GCTGAGT54400.017.50288212
Produced by FastQC (version 0.11.2)