FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24404859
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTG301950.12372536141265966No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG283730.11625963501776429No Hit
CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCC256830.1052372398463765No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT254960.10447099899245475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42500.035.1482315
CACGTCT45400.033.35955414
ACACGTC50850.030.39494113
CCCGAAT36050.030.24297735
CGAATCG33300.029.21708937
GTCACTC40300.026.96763629
CGGGACT40700.025.516131
GTCTGAA62950.024.55194317
ACATGCG54850.023.148264
GCCAGTA88650.022.9234141
ATGCGTG55800.022.8779186
TCACTCC47900.022.6889430
CAGTCAC48100.022.59358227
AGTCACT47600.022.54108628
CATGCGT59450.021.5312945
CTTAAAC70300.020.6618651
GTTAGTT74500.020.3306371
CACTCCC54600.019.7786331
CTACCCG52950.019.6132496
CGCTATG96800.019.42488929
Produced by FastQC (version 0.11.2)