FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bw_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bw_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9888829
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG17200.036.3031515
CACGTCT19600.032.21037314
ACACGTC24200.026.37285413
CTCGTAT14700.024.40923142
TCGTATG16700.022.9319143
CGTATGC15600.022.77949744
TATGCCG15750.022.3432746
GTCACTC20150.022.08792729
CGGGACT19500.021.4093881
CAGTCAC21350.021.00720227
AGTCACT21900.020.63736728
GTCTGAA31400.019.99576817
CTTAAAC25550.019.5808071
GCCAGTA35650.019.5499341
CGCTATG34300.019.41348529
GTCGCTA33600.019.30373427
GTTAGTT29000.019.2739411
ATGCGTG24700.017.880416
CGGAATA21750.017.60641968
ACATGCG25150.017.1487354
Produced by FastQC (version 0.11.2)