FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir64_3bs_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir64_3bs_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15499057
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG28200.035.10994715
CTTATCG21400.029.5037237
ACACGTC34500.029.19901513
CACGTCT37750.026.68527814
AGTCACT33350.026.48323228
CAGTCAC37350.024.0162627
TTATCGG19400.024.00893438
CGGGACT27350.023.8419151
CGCTATG59250.020.61375829
GTCTGAA49400.020.601217
GTCGCTA60000.020.35516527
CATGCGT34300.017.9049785
ACATGCG35150.017.8645114
GGTAACT69500.017.32376569
ATGCGTG36850.017.0405866
AGCACAC60350.016.97826810
GCTATGA73450.016.72252530
CGCGAAT38850.016.6956561
CACACGT60450.016.60748912
CTACCCG39500.016.0720546
Produced by FastQC (version 0.11.2)