FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8620958
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10500.041.07282643
ACGTCTG17400.040.05076615
CTCGTAT11050.037.46777342
TATGCCG12450.033.2531846
CACGTCT21550.032.97928214
ACACGTC22150.032.3972713
CAGTCAC19250.029.0342527
CGTATGC14950.028.1544844
CTAATCT17350.028.0385837
CGGGACT17900.027.369491
GTCTGAA26450.026.47762517
ACATGCG19950.025.7677964
CATGCGT19900.025.6591645
ATGCGTG20850.024.986466
TAATCTC18550.024.73697938
AGTCACC22150.024.61003528
GAGCACA33550.022.9312939
GTATGCC19600.022.00342845
AGCACAC34550.020.96934910
TGCGTGC25800.019.7899937
Produced by FastQC (version 0.11.2)