FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20786689
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35600.039.1529443
CTCGTAT35600.038.959342
ACGTCTG45750.037.4768215
TATGCCG43650.032.2486146
CGTATGC44400.031.93660444
CACGTCT58600.029.78926514
ACACGTC61700.028.18066413
CAGTCAC57350.026.64967227
GTATGCC60550.024.04599845
GTCTGAA77750.022.40724817
ATGCCGT65900.021.3077147
CACACGT83250.021.13459212
AGTCACC73650.020.89217428
ATCGGAA85950.020.0708872
CGCTATG58700.019.39622529
GTCGCTA63150.018.13786927
TCGGAAG98050.017.5940533
CGGGACT34400.016.9499971
GAGCACA111650.016.9023069
GCACACG106250.016.75447511
Produced by FastQC (version 0.11.2)