FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18245611
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23450.040.7534344
TCGTATG24400.039.450745
ACGTCTG36500.036.8610815
CACGTCT43750.031.22613114
CGTATGC32800.030.29333546
TATGCCG30650.030.0537448
ACACGTC47350.029.28941513
CACAGTC37750.028.8811231
AGTCACA38800.028.27599328
CAGTCAC39900.027.92863727
GTCACAG42450.025.84571529
TCACAGT45150.023.84176430
GTCTGAA58050.023.41485617
ATGCCGT39550.022.94183749
CGGGACT37200.022.3528181
GTATGCC45650.022.2190547
ACAGTCA50400.021.6319532
AGCACAC68150.020.09718710
CGCTATG67750.019.45329729
ACATGCG40100.019.1875654
Produced by FastQC (version 0.11.2)