FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23499910
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT267520.11383873385047007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG35300.046.13177533
GTCACGT40100.040.5236429
ACGTCTG48800.039.3057815
ACGTTTC41300.038.84513532
TTTCGGA42750.038.09324635
CACGTTT45050.035.91812531
AGTCACG45350.035.52737828
TTCGGAA46650.034.98211336
CACGTCT56800.033.95239614
ACACGTC57750.033.51320613
TCGGAAT49950.032.3947637
CGGGACT53250.028.8325751
CAGTCAC57450.028.1044327
GTCTGAA75700.025.97648417
ACATGCG57850.022.4850544
ATGCGTG63250.020.7838466
AGCACAC95850.020.73221210
GAGCACA99950.020.1926359
CATGCGT64000.020.1088225
CGCTATG103150.020.10190229
Produced by FastQC (version 0.11.2)