FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19947352
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT83850.050.98124329
ACGTCTG93650.047.77855315
TCACGTG89900.047.47371730
AGTCACG91450.046.70528428
GCCTTAT93850.044.99725737
CTCGTAT52950.044.37112844
ACACGTC104500.043.1481613
CACGTCT104800.043.12339814
TCGTATG53050.040.77641745
CAGTCAC105800.040.4356127
CCTTATC101700.040.3704738
CGTATGC55300.039.67823846
CACGTGG110250.038.77387631
CACACGT116800.038.5455212
TATGCCG57950.037.92294348
TATCTCG72800.037.4878541
GTCTGAA124000.036.41831217
ATCGGAA129200.034.716162
ACGTGGC126900.034.01244432
CTTATCG29700.033.92124639
Produced by FastQC (version 0.11.2)