FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10133110
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT116320.11479200363955389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16300.047.41342
TCGTATG17150.045.2642543
ACGTCTG22500.041.55176515
TATGCCG19250.039.42941746
CACGTCT25050.037.3223114
CACCTAT22750.035.0320431
CAGTCAC23150.034.72274827
ACACGTC28100.033.76257313
CGTATGC23650.033.55235344
TCACCTA24100.033.3556530
AGTCACC25100.032.0253228
CTATACA26350.030.37696334
CGGGACT22300.029.8604181
GTCTGAA31850.029.57031417
ATGCCGT26000.029.32490547
GTATGCC27700.028.8960745
AGCACAC36350.026.47957810
GAGCACA38700.025.3176239
TATACAT33400.023.96529635
GCCGTCT31400.023.07317449
Produced by FastQC (version 0.11.2)