FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bw_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bw_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12111009
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT128630.10620915235055972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG33100.045.12865415
CACGTCT36550.041.15257314
ACACGTC38150.039.42680713
CTCGTAT17200.038.71319242
TCGTATG17700.037.81405343
CAGTCAC36950.036.32042727
TCACCTA39550.034.02221330
AGTCACC39350.034.01795228
TATGCCG19600.033.79705446
GTCTGAA45450.033.09395617
TATCTCG23850.031.97068839
CGTATGC21900.031.1919344
CACCTAG44100.030.82505831
AGCACAC54050.028.08454510
CCTAGCT49300.027.92307733
CTATCTC28350.027.74765638
GAGCACA58500.026.2433919
CGGGACT29600.025.7601641
CACACGT58900.025.53737312
ACATGCG26500.024.347614
Produced by FastQC (version 0.11.2)