FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bs_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bs_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14938337
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41150.040.74405315
CTCGTAT32050.039.72132542
TCGTATG35500.037.90170343
CGTATGC34150.037.379544
ACTGACC42750.035.10706332
ACACGTC49250.034.25359713
CACGTCT50650.033.51114314
TATGCCG38200.033.32673346
AGTCACT47400.031.37039228
CAGTCAC50150.029.92515827
GTATGCC49000.026.40388545
GTCTGAA65350.026.1311717
CTGACCA60600.025.33528933
CGGGACT30100.025.1031231
CACACGT67900.024.89610112
ATGCCGT50900.024.19755447
AGCACAC73700.023.82655110
ACCAATC64400.021.69754236
GAGCACA84750.020.801869
ATCGGAA82650.020.6638372
Produced by FastQC (version 0.11.2)