FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_4bs_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_4bs_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24710506
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT52900.035.02300642
TCGTATG55450.034.7809643
CGTATGC56000.034.62376844
ACGTCTG68000.033.53466415
TATGCCG65250.029.503846
ACACGTC80550.028.8237813
CACGTCT83850.027.56607414
AGTCACA82000.024.44485328
CACACGT94300.024.40139612
TCACGAT85250.024.12052235
CAGTCAC85200.023.85044327
ATCACGA87850.023.72045134
GTATGCC85500.022.7990745
ATCGGAA107550.021.6545562
ATGCCGT90650.021.31286647
GTCTGAA109050.021.22755617
CACGATC92200.020.58054736
GTCACAT102250.019.70551729
TCGGAAG122950.018.717923
CGTCTGA125000.018.57404516
Produced by FastQC (version 0.11.2)