FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bw_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bw_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7202505
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11850.048.0377242
ACGTCTG21850.044.99819615
TCGTATG12300.043.4759843
ACGATAT22000.039.04995735
CACGTCT25700.038.65966414
CAGTCAC22850.038.65321727
TATCTCG17000.037.95164539
ACACGTC26350.037.70574613
CCACGAT23750.037.18949533
TATGCCG14450.036.5297246
AGTCACC25100.035.32647728
CACGATA25600.034.36696234
CGATATC24900.033.669936
CGTATGC16350.032.91748444
GTCTGAA31050.031.9989417
ACCACGA28600.031.0039532
CACACGT32900.029.88461312
ATATCTC23300.028.5782938
ATCGGAA36800.026.8134752
AGCACAC37450.026.71473710
Produced by FastQC (version 0.11.2)