FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bw_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bw_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23014902
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT241620.10498415331075492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG47400.042.35898615
CACGTCT57600.035.39710614
ACACGTC61900.032.7709113
CAGTCAC57700.030.31427827
CGGGACT47950.026.9111941
AGTCACC65600.026.66379728
GTCTGAA76650.026.59972417
ACCGGAT71900.023.99289135
AGCACAC89950.023.3577610
CGCTATG85350.022.0303929
ACATGCG50700.021.845464
CACACGT95500.021.34945912
GTCGCTA88850.021.20070327
GAGCACA101900.021.1942949
ATGCGTG53600.021.0499386
CATGCGT53600.020.728035
GATCGGG81350.019.72127339
ATCGGAA106200.019.1031342
GCTATGA101300.018.66385330
CTCCAGT110550.018.66152624
Produced by FastQC (version 0.11.2)