FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bw_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bw_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11721325
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23350.048.1679544
TCGTATG23800.046.82372345
ACGTCTG31650.045.5623315
CACGAGT28900.042.8586931
TATGCCG26150.041.55822848
CGTATGC27650.039.80331846
TATCTCG29700.039.3815641
CACGTCT37600.038.81175614
AGTCACG31650.038.8069328
TCACGAG31900.038.5032930
ACACGTC40300.036.3823713
CAGTCAC34750.036.0395427
GTCACGA35900.034.69375629
ACGAGTG35550.034.64697332
GGATATC36750.032.95179438
GTCTGAA45400.032.29508617
GTATGCC35350.031.32808347
ATATCTC40150.029.99103240
GATATCT41750.028.75866539
ATGCCGT38200.027.99732449
Produced by FastQC (version 0.11.2)