FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bw_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bw_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12709309
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC17800.038.9601438
TACGTAA19600.038.5507236
ACGTCTG25550.034.97120715
ACGTAAT22750.033.06127537
CCGTACG24050.030.69997633
CTCGTAT14450.029.60563744
CACGTCT31100.029.28500614
TCGTATG14150.028.52749445
ACACGTC34400.027.17758813
CAGTCAC28650.027.09422527
CGGGACT28600.024.9720341
TATGCCG15600.024.76931648
GTCTGAA40200.023.08489817
AGTCACC34600.022.1359128
TAATCTC26600.020.10487640
ACCGTAC39850.020.08657332
CGTATGC19500.019.9926146
ATGCGTG30250.019.3901736
ACATGCG30000.018.7466134
CATGCGT30300.018.5615
Produced by FastQC (version 0.11.2)