FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bw_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bw_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421857
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCA95210.12828325848908165No Hit
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA86210.1161569132900297No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT82410.11103690087265222No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC81810.1102284778593821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG27500.053.9460928
CTCGTAT15650.052.24662442
ACGTCTG31100.051.24899315
GTCACGA29550.049.9714829
CAGTCAC30550.049.01170727
TCGTATG17250.048.20024543
ACACGTC33550.048.0213113
CACGTCT34350.046.7020314
TATGCCG17850.045.03414546
GTCTGAA35900.044.5889517
CGTATGC19500.042.815644
ACGACAT35100.041.67711335
AGCACAC42750.037.6871510
GAGCACA43400.037.5204249
CACACGT43000.037.46778512
CTGAACT43900.036.8565819
ATGCCGT21300.035.309847
GTATGCC24200.034.6436745
GACGACA43050.034.62085334
TCTGAAC48000.033.4209218
Produced by FastQC (version 0.11.2)