FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bw_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bw_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11123475
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT118780.10678317701977125No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC114050.10253090873130924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT30250.050.18367429
ACGTCTG35650.049.35311515
CTCGTAT22600.049.00269744
CACGTGA31500.048.08294731
AGTCACG32500.047.66333828
TCACGTG32700.046.5294530
ACACGTC38650.045.61180513
TATGCCG23750.045.1764848
TCGTATG25350.044.50360545
CACGTCT39900.044.44225314
ACGTGAT34250.044.42354232
CAGTCAC39200.039.42843227
CGTATGC28000.038.68914846
CGTGATC39950.038.25798433
GTCTGAA47300.037.4164117
ATGCCGT32000.031.80457749
GAGCACA56650.031.7892559
CGGGACT30300.031.3134571
CACACGT56600.031.08562512
GTATGCC35800.031.03020147
Produced by FastQC (version 0.11.2)